Seeds From Doug Keel

Do something similar for Scott Armstrong's data and then put a message on Pumpkins, Genetics, and Eddleman, Bioteach and etc to publish these URLs

Here put a black paragrphy summarizing prior work call it iSi, summary of prior work referring to scott armstrong data

Doug Keel's Initial Report of his White/Tan Data

Here is the initial letter from Doug Keel. Below this letter Harold Eddleman discusses each mating with Eddleman's words in blue color.

Subject: Seed color
Date: 10 Apr 99
From: dwkeel@sprint.ca To: Indbio

Here is a little information about my 662 Keel '97.

662 Keel'97 (w seed) -- (F) 805.5 Eaton'96 ( T seed) X (M) 742 Glasier'96 ( Tseed) ----------------------------------------------------------------------------------------

465 Keel'98 ( W Seed ) -- (F) 662 Keel (W Seed) X Self -----------------------------------------------------------------------------------------

508 Keel'98 (W seed) -- (F) 662nKeel (W seed) X (M) 500+ LA Rue'97 ( T seed ) -----------------------------------------------------------------------------------------

555.5 Eaton'98 ( T seed ) -- (F) 662 Keel ( W seed ) X (M) 853 Eaton'97 ( T seed ) -------------------------------------------------------------------------------------------

572 Wentzell'98 (T seed ) -- (F) 662 Keel ( W seed) X (M) 606 Wentzell'97 ( W seed) ------------------------------------------------------------------------------------------

Hope this helps. As you can see the offspring from the same parent (662Keel) produiced white and tan seeds. I would like to hear your thoughs on this. All the best
Doug Keel

Eddleman's Interpretation of Keel's Data

Eddleman's words are in Blue. This section is not completed; Doug Keel and Eddleman are still discussing the data.

Introduction

Eddleman's interpretation of Keel's Data. Eddleman is assuming no mutations occurred during the experiment because mutations usually occour only about 1/1,000,000. Since all seeds in a fruit are the same color, we assume the seed coat is maternal tissue and depends upon the genotype of the mother.

From Scott Armstrong's data we developed the hypothesis that "If a fruit has white seeds the mother is homozygous for the white allele and is tt. Any fruit having tan seeds is Tt or TT." We will now examine Doug Keel's data for fit to this hypothesis.

The Hypothesis

Here spellout the details of the Hypothesis in simple terms so everyone understands it.

I lack time now but t represents the recessive allele for white and T represents the dominant allele which produces tan color. If you have seen tan and white seeds, you have noticed white seeds are thinner, have a rim and usually fine wrinkles. All this suggests more than color is involved. When I get some "throw away" seeds, I will dissect hoping to find the structural differences.

Harold's Intrepretation of each Mating

Here is a little information about my 662 Keel '97.

662 Keel'97 (w seed) <== (F) 805.5 Eaton'96 ( T seed) X (M) 742 Glasier'96 ( T seed)

According to the hypothesis, 662 Keel is homozygous ( tt )for white seeds.
Thus, each parent contributed a white allele to 662 Keel.
Therefore, 805.5 Eaton 1996 and 742 Glasier 1996 are each Tt genotype.

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465 Keel'98 ( W Seed ) <== (F) 662 Keel (W Seed) X Self

Here a tt plant was selfed and produced tt offspring as expected by the hypothesis. We will want to save some of the seed of 465 Keel as our proved homozygous white seed for used in matings, testcrosses, and other uses.

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508 Keel'98 (W seed) <== (F) 662nKeel (W seed) X (M) 500+ LA Rue'97 ( T seed )

This fits the hypothesis. LaRue was probably Tt and contributed a t sperm to that 508 Keel is tt and therefore white.

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555.5 Eaton'98 ( T seed ) -- (F) 662 Keel ( W seed ) X (M) 853 Eaton'97 ( T seed )

Since 853 Eaton is tan, the hypothesis assumes it is TT or Tt . 555.5 Eaton should be Tt .

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572 Wentzell'98 (T seed ) -- (F) 662 Keel ( W seed) X (M) 606 Wentzell'97 ( W seed)

Notice two white seeds mated gave a tan seed. This is unexpected and does not fit the hypothesis. There are easy possibilities.

1) contamination. Perhaps the male flower bag was not insect thight and some brown pollen got in.

2) We have two different whit loci. That is not too unusual.

3) Perhaps 606 Wentzell had brown seeds and the above mating says white seed by error.

4) Reversion of the white allele to tan; rare but posible. There are other rare explanations.

Doug you may be able to save years of work by finding some error in the Wentzell mating you wrote above.

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Hope this helps. As you can see the offspring from the same parent (662Keel) produiced white and tan seeds.

The Fault with the Above Data

To some extend the above data were selected to prove the point. The above were only a tiny fraction of the seeds produced by these plants. To be honest, one would like to examine every seed but that requires planting every seed in a fruit to see what color seeds it produces.

You may send private e-messages to Dr. Eddleman and he will reply, usually within 24 hours.

Re: Keel Data Page (dwkeel@sprint.ca, 04/21/99 06:56) To: Indbio

Dear Harold; I believe this info, You show 606 Wentzell x 992 Keel giving tan should be, 572 Wentzell'98 --- (F) 662 Keel'97 white seeds X (M) 606 Wentzell white seeds --- Giving tan seeds. I do not know if Roger Wentzell covers male blossoms but I will check it out and get back to you. Last year (98) I started covering my male blossoms. I have a 606 Wentzell and 555.5 Eaton'98. Will send them along with other seeds for you. --------------- Doug -----Original Message----- From: Harold Eddleman Ph.D. <indbio@disknet.com> To: dwkeel@sprint.ca <dwkeel@sprint.ca> Date: Wednesday, April 21, 1999 2:18 AM Subject: Keel Data Page

>Dear Doug > I uploaded pk718 > >Look at the page and check the last mating you sent me. You show 606 >Wentzell x 992 Keel giving tan. Not impossible but unlikely. > I treasure my exceptions, that is where the discoveries are made. The >first question is whether 606 Wentzell is really white seeded. If so, >then was the bag to keep insects off the male flower tight enough to >prevent insects carrying tan pollen onto the flower. I suspect most >people do not bag he male, I always do. > >The URL for our joint work is below > >Harold Eddleman >14045 Huff Street >Palmyra IN 47164 >-- >Harold Eddleman Ph.D. Microbiologist. mailto:indbio@disknet.com >Location: Palmyra IN USA; 36 kilometers west of Louisville, Kentucky >http://www.disknet.com/indiana_biolab/pk718.htm > > > >