The main yeasts
of medical in interest are Candida albicans and Cryotcoccus.
For uniformity, I am using the Bacteria site as a model for the Yeast
pages. I am still making the needed changes. Only yeast pages listed above
this line are installed at present.
This site has the information to enable any young Pasteur to conduct
excellent microbiology experiments at home with materials found in any
kitchen. Farmers, gardeners, and amateurs will find useful methods and
information on these pages for science projects, family health, gardening,
farming, animal care, and food preservation. Inexpensive kits of Cultures
and supplies are available.
First-time visitors please click here for information
moved from this page to make downloading faster. For fast browsing,
Bookmark this page, then use your Back Button
to return to this index as you finish looking at any page of this site.
Start Your Microbiology Science Project Here, Today!
B000 - Index page giving general safety
and background information for your microbiology project. This page will
help you get an outstanding bacteria project going. Also visit our yeast,
fungi, and bacteriophage
web sites which are in early construction.
- B001 - Letter to the Beginning Student
- Read before trying any of these experiments.
- B002 - Letter to Parents - This letter
discusses the safety risks of bacteria projects.
- B003 - What is microbiology?
What are bacteria, archeobacteria, viruses, fungi, prions?
- B004 - Introduction to bacteria:
their food and growth conditons; their place in bioworld
- B005 - BacteriaStudyList - mail list
for K-12 students, parents, farmers, home makers, et al.
- B006 - Believe it or Not. Surprising,
but true facts about bacteria. INCOMPLETE
- B007 - Bacteria: Friends or Foes?
- - plasmids, genetics, phages, (introductory info every beginner should
know for safety).
- B??? - More basic information about bacteria
- - more beneficial bacteria
- - some harmful bacteria
- books, magazines about bacteria
- short history of microbiology
- how bacteria got their names
- links to bacteria sites
- into to growth requirements of bacteria.
- B017 - Suggested gifts
for your young Pasteur--some are free.
- bacteria on and in our bodies - could we live without them?
- B018 - Reports on bacteria projects completed by K-12 students.
- B019 - Sample Student Research Proposal
- planning your project before you begin
B020 - Start your own Home Microbiology
Lab by reading this index page. Introductory experiments, media, tools,
and methods for beginners using foods and items found at home--few or no
purchases required. B020a prints a work
plan for starting your bacteria project.
- B021 - An easy non-sterile first
experiment. Can yeast use corn syrup, sugar, or starch?
- B022 - Make E-Broth from ground beef
on the kitchen stove - Easy-meat broth medium.
- B023 - Dispense your media as stabs,
slants, plates, deeps: aerobic and anaerobic media
- B024 - Sterilize and store your media.
Use pressure cooker or steamer; store dust-free
- B025 - Make a loop and streak gelatin
or agar plates to isolate pure colonies. Methods.
- B026 - Start your own pure cultures
collection. Make chart of bacterial
- B027 - More meat-based media: liver,
egg, bouillon cubes, gelatin, heart infusion, brain
- B028 - Milk media. Many bacteria
grow better in milk than in meat broth. A
- B029 - Make media from potato, carrot,
tomato, rice, hay, turnip; slices, plugs, and liquid
- B030 - Stock Cultures Media - as
used at Indiana Biolab. Study this important page.
- B031 - Chemolithotropic media: rocks
and sulfur; mud jars. For bacteria that eat rocks.
- B032 - Simple diagnostic media which
can be made at home. Gas tubes, sugars used.
- B033 - Growth conditions; Aerobic
vs anaerobic bacteria; media, incubation, and methods.
- B034 - Introduction to enrichment
and selective media for growing specific bacteria.
- B037 - Reuse plastic plates and trays.
Experiments using bacterial lawns. Reuse & recycle.
- B038 - How to count bacteria.
Sub pages on statistics, equipment, simple to complex
- B039 - Summary of tools, equipment,
and media for your Home Micro Lab. Incomplete
B040 - Index page to Standard Methods
used in Microbiology: This chapter shows you how to perform the techniques
used in colleges, hospitals, and professional laboratories. The amateur
microbiologist may not have access to this equipment, but these pages help
him design or select substitutes so that he is able to complete many additional
- B041 - What are agar, tryptone, yeast
extract, and the other ingredients used in media?
- B0?? - Preparing professional media from tryptone, yeast extract, agar
grades, origin of
- B0?? - Formula for professional media
- B042 - Formula for diagnostic media
- carbohydrate media
- B0?? - Formulae for bacteriological stains
- B0?? - Streaking plates to isolate bacteria and obtain pure cultures
- B0?? - Making slide mounts and using microscope
- B0?? - Building and maintaining a collection of bacteria generally
- B0?? - ?building equipment for advanced work, laminar flow hoods, safety
- B0?? - working with strict anaerobic such as rumen bacteria and methane
B060 - Experiments on Growth Requirements of Bacteria. Effect of environmental
factors on the growth of bacteria. Performing these experiments will help
you understand bacteria and how to protect your food from spoilage and
poisoning by bacteria . Salt, sugar, pH, oxygen level, heat, and other
agents are all used to protect and preserve foods. In these experiments
you will aloso learn how different genera and species of bacteria differ
in their response to environmental conditions.
B090 - Beginner's Guide to Classification
of the Funi NOT INSTALLED
- B061 - Oxygen requirements of bacteria
- B062 - Optimum temperature for some
common bacteria: 4C, room temp, 37C, 55C
- B063 - Osmotic pressure (salt) of
medium and bacteia - INCOMPLETE
- B06? - pH effect on bacterial growth. Table: species, comments
- B064 - Nutritional requirements of bacteria
- B065 - Carbohydrates used by bacteria - diagnostic assays Henry page
- B0?? - Carbon sources used by bacteria
- B0?? - Nitrogen sources used by bacteria
- B000 - Disinfectants action on bacteria
- B000 - Killing of bacteria by UV, heat, and agents is a logarithmic
function; killing curves.
- B000 - chemo- and organo- tropic nutrition
- B000 - Bacteriophages (viruses that attack bacteria). Also see phage
- B000 - Bacteriocins that kill bacteria
- Growing bacteria on minimal media; streak 10 species on a minimal plate.
F100 - Food Bacteriology: Experiments
with the fungi you eat every day NOT READY
F200 - Isolation of fungo from nature
based on their traits.Not Installed
F400 - A Professional Classification
of the Bacteria. INCOMPLETE
F800 - Medical Mycology; move pathogenic
discussions here - not ready (incomplete)
- B801 - First fungi safety page for beginners - required reading for
- B802 - Safety page for High School and Begin College students
- B??? - lists of pathogenic bacteria class 1,2,3, 0=no report of disease
- B0?? - The pathogenic bacteria: disease causing bacteria, plants and
animals, microbiol. safety
F900 - Bacterial genetics INCOMPLETE
First time Visitors Please
You can easily and safely run a bacteria project at home for a few years.
Such long-term projects usually win college scholarships for young scientists.
These pages were written for the young Pasteur who has an interest in
microbes, but has no books, no equipment, no microbes--nothing but a desire
to learn. You will find protocols for isolating bacteria from nature, descriptions
of species, and useful information not found elsewhere on the Web. With
this information you will be able to start you own collection of pure cultures
and identify many of them, at least assign them to the correct genus. If
you put in that much effort, you will probably learn as much as many students
in a good college microbiology course. Likewise elementary and highschool
teachers, farmers, gardeners will learn much to aid them. Eventually this
site will be 20 times as large as it is today.
You do not need a microscope. Toy microscopes are useless for studying
bacteria. A microscope good enough to see bacteria costs hundreds of dollars.
You would have to pay over $1,000 for a microscope that would be really
useful. If you want to see your bacteria, take them to school and use a
microscope there. Instead of spending money on a microscope, it is better
to concentrate on isolating bacteria from nature and studying their biochemical
traits. Someone once told Pasteur he had called a bacterium a coccus (round)
when it was actually rod-shaped. "If you only knew how little difference
that makes to me," Pasteur replied.
There is no need to go out and buy lots of equipment. Begin with Home
Micro Lab by making media from common kitchen foods and isolate some
Bottles can serve as culture tubes, or your teacher may loan you culture
tubes and supplies. If not try a hospital, they throw away lots of items
you can use and if autoclaved they are perfectly safe. Before you trouble
a hospital for gifts, do a few week's work at home and prove that you are
really interested and planning to work hard. Can lids, or flat bottles
can serve as petri plates. Most students only have access to plastic petri
plates, but is sometimes possible to reuse them.
BA.htm - Isolation and study of Bacillus
CL.htm - Isolation and study of Clostridum
Some day there will be pages on yeasts and fungi.
You may send private
e-messages to Dr. Eddleman and he will reply, usually within 24 hours.
First installed January 1998 Revision
#6 1998 March 12 firstname.lastname@example.org
Written by Harold Eddleman, Ph. D., President, Indiana Biolab, 14045 Huff
St., Palmyra IN 47164
| Indiana Biolab
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Evironmental Needs | Food Microbiology
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