Isolation of Pure Cultures Of Bacteria

Isolation of Desulfovibrio from anaerobic muds

The Desulfo-bacteria reduce sulfur, sulfates, or other sulfur compounds to H2S.

Bergey's Manual of Systematic Bacteriology (Vol 1. page 666. (1984)) has additional information. This genus has 9 species which differ considerably in size and biochemical capabilities. Beginners should find it fairly easy to isolate strains of this genus in any geographic area. They are found in the anaerobic bottom muds of freshwater, brackish, and marine sites.

Click on Genus Desulfovibrio for the diary of an attempt to isolate bacteria of this genus.

II. Bacteria capable of dissimilatory sulfate reduction. Sulfite, thiosulfate, or other oxidized sulfur compounds may also serve as electron acceptors. Dissimilatory sulfate-reducing bacteria

1. Cells are rod shaped, helical, vibrioid, or spirilloid, occasionally straight and are motile. Incomplete oxidation of lactate to acetate and carbon dioxide is common. Some strains use fatty acids that may be oxidized completely. Genus Desulfovibrio

Media for Desulfovibrio species.

Component

Concentration, grams/ liter

Medium B Medium C Medium D
KH2PO4 0.5 0.5 0.5
NH4Cl 1.0 1.0 1.0
CaCO4 1.0 --- ---
Na2SO4 --- 4.5 ---
MgSO4 . 7  H2O 2.0 2.0 ---
Sodium lactate 3.5 3.5 ---
Sodium pyruvate --- --- 3.5
CaCl2 . 2H2O --- 0.06 0.1
MgCl2 --- --- 1.6
FeSO4 . 7 H2O 0.5 0.004 0.004
Yeast Extract 1.0 1.0 1.0
Sodium citrate --- 0.3 ---

Web Sites of Interest

If you have any questions send me an email indbio@disknet.com    Let me know how this experiment worked for you.


First installed Sep 1999      Revision #1 1999 Sep 27           H2SO4     1022m
Bacteria Index Page | INDIANA BIOLAB Web Sites | indbio@disknet.com
Written by Harold Eddleman, Ph. D., President, Indiana Biolab, 14045 Huff St., Palmyra IN 47164