Isolation of Pure Cultures Of Bacteria

Sulfur-reducing Bacteria = Desulfo-species

The Desulfo-bacteria reduce sulfur, sulfates, or other sulfur compounds to H2S.

In the 1984 issue of Bergey's Manual of Systematic Bacteriology (Vol 1. page 663) there are 9 genera of sulfur-reducing bacteria. Some reduce elemental sulfur and other reduce sulfur compounds. They all produce hydrogen sulfide. Hydrogen sulfide is the gas which gives rotten or boiled eggs their characteristic odor.

Click on Genus Desulfovibrio for the diary of an attempt to isolate bacteria of this genus.

Key to the sulfate- and sulfur reducing bacteria genera

I..  Bacteia able to oxidize acetate completely to CO2 using elemental sulfur as the electron acceptor. Sulfate is never reduced. Dissimilatory sulfur-reducing bacteria.GenusDesulfuromonas

II. Bacteria capable of dissimilatory sulfate reduction. Sulfite, thiosulfate, or other oxidized sulfur compounds may also serve as electron acceptors. Dissimilatory sulfate-reducing bacteria

1. Cells are rod shaped, helical, vibrioid, or spirilloid, occasionally straight and are motile. Incomplete oxidation of lactate to acetate and carbon dioxide is common. Some strains use fatty acids that may be oxidized completely. Genus Desulfovibrio

2. Genus Desulfomonas

6 more genera to be installed later.

Web Sites of Interest

If you have any questions send me an email    Let me know how this experiment worked for you.

First installed January 1998      Revision #7 1999 Sep 9   H2SO4     1022m
Bacteria Index Page | INDIANA BIOLAB Web Sites |
Written by Harold Eddleman, Ph. D., President, Indiana Biolab, 14045 Huff St., Palmyra IN 47164