Chart of Bacteria Traits

Once you have isolated some bacteria in pure culture (restreaked to make sure they are pure) or have obtained known pure cultures from a professional, you should run assays on the strains using professional media or media you have prepared from what substances you have available. Then you should make a chart similar to this one to record the results. If possible print this chart as a guide, but when you make your own chart enter the tests which you ran. On a second page describe your medium and how you ran the assay. Keep a bench sheet as you work so that you can later recall exactly how you ran the test, temperatures, water used, etc. The second table gives the species used to prepare the first chart. blank cells indicate assay not yet completed.

Assay                  Species ==> 1 2 3 4 5 6 7 8
Gram Stain; + = violet color - - + - + + - -
Cell Shape and arrangement _ o _ _ _ ooo _ s
Motility; + = motile + o + + + o + +
Spore Formation; spores = + o o + o + o o o
Capsule formation = + slime
Catalase formed = + + + + + o o + +
Gelatin digested 24hr @37C o p + o o o o o
Gelatin digested 72 hr @ 37C o p + o o o o o
Starch digested = + o o + o + o o o
Growth on TGY agar @ 45C +
Growth on TGY agar @ r.t. + + + + + + + +
Growth on TGY agar @ 5C
Growth in shake culture 30C F T T F A F F M
Glucose fermented = +
  acid = Y
+
Y
o o + + o +
Y
o
Sucrose fermented = +
Lactose fermented = +
Hydrogen sulfide produced = + +
Urease reaction; red = + o o +? +
Ulrich milk; coagulate = + + o + + o o
Ulrich milk; peptonized = + o o + o o o +
Ulrich milk; methylene blue
  reduced = +
+ o o + o +
Ulrich milk; made alkaline = + o o o o o +

The table below lists the species used in the above table

1. Escherichia coli B 5. Clostridium felsineum
2. Micrococcus roseus 6. Leuconostoc mesenteroides
3. Bacillus megatherium 7. Proteus vulgaris
4. Serratia plymuthicum 8. Rhodospirillum rubrum